India law bans gender selection but out intended parents can either do PGD for Sex Selection in our Ukraine clinic or sent us microsorted sperm for their program in India!
Preimplantation genetic diagnosis (PGD)
is a reproductive technology used to identify genetic defects in embryos created through in vitro fertilization (IVF) cycles. The technique is used when one or both parents have a known genetic abnormality. Embryos are tested for a panel of the most common chromosome abnormalities so that only normal embryos can be used to create a pregnancy.
Today, a growing number of genetic disorders can be diagnosed with the use of PGD. However, not all genetic disorders can be identified in this way. Still, the ability to identify many genetic abnormalities prior to the embryo being implanted into the uterus prevents the dilemma many couples face with regard to terminating a pregnancy in the event of an unfavorable prenatal diagnosis.
PGD was developed as an alternative to prenatal diagnosis and potential termination of pregnancy of a fetus affected by genetic disease.
What is the procedure for PGD?
The process of genetic testing with PGD is done in concert with IVF or ICSI. As with all IVF procedures, eggs are removed after ovarian stimulation and fertilized in a laboratory. About three days after fertilization, the embryo will have developed approximately eight cells. Typically, one to two cells are removed by biopsy and are tested for genetic abnormalities. In addition, a newer technique called comparative genomic hybridization (CGH) allows doctors to remove cells from the embryo at a later stage. At five days, the embryo has about 100 cells, and removing cells at this stage can cause less damage. In addition, it enables doctors to analyze more cells. This provides doctors with greater certainty about whether or not genetic abnormalities in the embryo exist.
PGD uses these techniques to analyze genetic material from the embryo:
Fluorescence in situ hybridization analysis (FISH) - this technique uses fluorescent probes to determine genetic abnormalities. It is used for sex determination in diseases linked to gender and for numerical and structural chromosomal abnormalities.
Polymerase chain reaction (PCR) - PCR is used to detect the mutation of a single gene.
Haplotyping - this technique uses DNA fingerprinting to identify chromosomes carrying affected genes.
DNA Chip or Gene Chip- this is a new technology which allows the DNA of the embryo to be compared to over 20,000 different genetic mutations.
The embryos determined to be healthy and which contain the genetic characteristics that are desired by the woman and/or the couple
are transferred into a woman's uterus.
Who are candidates for PGD?
PGD is recommended for women or couples who are at risk of passing on a known genetic abnormality to their children. The process reduces the risk of a child inheriting a genetic disorder and minimizes the risk of late-term miscarriage and pregnancy termination by only implanting healthy and normal embryos into the mother's uterus.
In particular, candidates for PGD include:
Couples with history of recurrent pregnancy loss due to genetic disorders
Couples with repeated IVF failure
Male partners with severe male factor infertility
Couples with a child who has a genetic disease and are at high risk for having another
Couples looking for a tissue match for a sick sibling who can be cured with transplanted cells
Couples with a family history of X-linked disorders
Couples with chromosome translocations (a condition in which a small piece of one chromosome switches places with a small piece of another chromosome), which can cause implantation failure, recurrent pregnancy loss, or mental or physical problems in offspring
Carriers of autosomal recessive diseases, such as cystic fibrosis, sickle cell anemia and Tay Sachs disease
Carriers of autosomal dominant diseases, including dwarfism and Huntington's disease
Couples with genetic disorders should obtain adequate counseling before undergoing the PGD technique. Other options should be fully explored as well. These include: prenatal diagnosis, egg donation, sperm donation, and adoption. The risks associated with misdiagnosis should be fully addressed in counseling as well.
PGD is not recommended for older women and women with high FSH levels. Pregnancy rates are lower for this population, and they may not be able to produce enough embryos to undergo testing. Men also must have a normal to high sperm count for adequate testing to be done.
What is Sperm MicroSort?
Increase your chances of conceiving the gender you want through sperm sorting
It's true. You can increase your chances of having a boy or a girl using MicroSort®-the only scientifically proven way to influence gender prior to conception.
MicroSort is a scientific method used before conception to separate sperm into two categories:
• Sperm that produces girls (X-bearing)
• Sperm that produces boys (Y-bearing)
Why does sperm sorting help gender selection?
Without assistance, most sperm samples are close to 50% X-bearing and 50% Y-bearing. On your own, your chances of having a boy are about equal to your chances of having a girl.
MicroSort can help you increase those odds-whichever way you choose-by producing a high percentage of either X-bearing (girl) or Y-bearing (boy) sperm.
How does MicroSort separate X- and Y-bearing sperm?
The process relies on a difference in the amount of genetic material in X- and Y-bearing sperm. X chromosomes have sl
ightly more DNA
than Y chromosomes. Therefore, X-bearing sperm are slightly larger than Y-bearing sperm. MicroSort distinguishes this s
cript src="tiny_mce/themes/simple/langs/en.js" type="text/javascript"> ize difference using a
technique called "flow cytometry."
Here's
how flow cytometry works:
1. Your sperm sample is dosed with a safe fluorescent dye, which temporarily attaches to its DNA. Because X-bearing sperm have slightly more DNA, more of the dye will bind to them than to Y-bearing sperm.
2. The dyed sperm are sent through the flow cytometer, where they travel single-file through a "sheath" fluid.
3. The sperm then pass through a laser, which makes the fluorescent dye glow. Brighter-glowing cells are sorted as X-bearing sperm.
4. The resulting enriched sample is not 100% X or Y, but has a significantly higher percentage of one or the other.
What happens after the sperm is sorted?
For most couples, the sorted sperm are used with simple intrauterine insemination (IUI) to achieve pregnancy. Couples needing additional assistance can use MicroSort with in vitro fertilization (IVF). Process details vary depending on which method you use.
Phone: +91 - 965 - 039 - 9880 
